v-SRC*

Protein v-SRC* map

Identifiers
v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
HUGO:SRC HGNC:11283 ENTREZ:6714 UNIPROT:P12931 GENECARDS:SRC REACTOME:402877 KEGG:6714 ATLASONC:SRCID448ch20q11 WIKI:SRC

Maps_Modules
 EMT  map  / EMT_REGULATORS  map
 EMT  map  / CELL_CELL_ADHESIONS  map
 EMT  map  / GAP_JUNCTIONS  map

References
PMID:16492141
Binding partners proteins of Connexin 43 (GJA1):
-Kinases: v-Src, c-Src, PKC, PKA, MAPK, Casein kinase 1, Cdc2 kinase
-TIGHT_JUNCTIONS scaffold proteins: ZO1, ZO2, caveolin 1
-Cytoskeleton: b-catenin, a-tubulin, b-tubulin
-Others: Drebrin, NOV, CIP85
PMID:9278444
Reduction of gap junctional communication in v-src transformed cells is accompanied by tyrosine phosphorylation of the gap junction protein, connexin 43
SH3 and SH2 domains of v-Src bind to proline-rich motifs and a phosphorylated tyrosine residue in the C-terminal tail of Cx43
PMID:9592087
Cx32 (GJB1) interacts with Cx26(GJB2), Cx46(GJA3), and Cx50(GJA8) but failing to do so with Cx40(GJA5).
PMID:15782139
Cx32 has a strong tumor-suppressive effect on a human metastatic renal cell carcinoma cell line.
Cx32-dependent inactivation of Src decreased the production of VEGF via the suppression of Stat3 activation

v-SRC*@Cytoplasm

References
e_re474( EMT  map ):
PMID:22315516
-Occludin is phosphorylated at S340 by PKC
-Occludin is phosphorylated at T383 and S508 by ROCK
-Occludin is phosphorylated at Y398, Y402 and Y474 by SRC
-Occludin is phosphorylated at T400, T404 and S408 by CK2
-Occludin is phosphorylated at S403 and S404 by PKC-N
-Occludin is phosphorylated at T424 and T438 by PKC-E
-Occludin is phosphorylated at S490 by VEGF
PMID:19017651
PMID:12604349
In mouse, phosphorylation of Occludin by SRC (at Y398, Y402) reduces TIGHT_JUNCTIONS assembly
In mouse, phosphorylation of Occludin by SRC (at Y398, Y402) reduces interaction between Occludin and ZO1,2,3
PMID:20152177
In mouse, phosphorylation of Occludin by SRC (at Y474) increases interaction between Occludin and PI3K
In mouse, phosphorylation of Occludin by SRC (at Y474) increases wound healing and migration
PMID:12547828
Inactive Src delays the oxidative stress-induced disruption of tight junction and accelerates calcium- induced assembly of tight junction in Caco-2 cells.
This demonstrates that oxidative stress-induced disruption of tight junction is mediated by the activation of Src.
e_re481( EMT  map ):
GJA1 is so-called Connexin 43 or Cx43
PMID:16492141
Binding partners proteins of Connexin 43 (GJA1):
-Kinases: v-Src, c-Src, PKC, PKA, MAPK, Casein kinase 1, Cdc2 kinase
-TIGHT_JUNCTIONS scaffold proteins: ZO1, ZO2, caveolin 1
-Cytoskeleton: b-catenin, a-tubulin, b-tubulin
-Others: Drebrin, NOV, CIP85
PMID:10871288
Interaction between Connexin 43 (GJA1) and PKC (alpha, betat and gamma subunits)
The 3 subunits phosphorylate GJA1 at Ser368 and reduce Gap junctions activity.
PMID:10679481
Fibroblast growth factor-2 (FGF-2)decreases cardiomyocyte GJ permeability by stimulating phosphorylation of connexin-43
FGF-2 activates receptors linked to PKC and MAPK
In immunoprecipitation experiments using specific anti-Cx43 antibodies, PKCE but not PKCA coprecipitated with Cx43.
FGF-2 increased levels of coprecipitated PKCE, suggesting increased association between PKCE and Cx43 on stimulation.
To conclude, PKC mediates the FGF-2–induced effects on cardiac GJs and that PKC likely interacts with and phosphorylates cardiac Cx43 at sites of intercellular contact
PMID:9278444
Reduction of gap junctional communication in v-src transformed cells is accompanied by tyrosine phosphorylation of the gap junction protein, connexin 43
SH3 and SH2 domains of v-Src bind to proline-rich motifs and a phosphorylated tyrosine residue in the C-terminal tail of Cx43
PMID:15534005
Phosphorylation of Cx43 on S368 has been shown to decrease gap junctional communication via a reduction in unitary channel conductance.
e_re548( EMT  map ):
GJB1 is so-called Connexin 32 or Cx32
PMID:9592087
Cx32 (GJB1) interacts with Cx26(GJB2), Cx46(GJA3), and Cx50(GJA8) but failing to do so with Cx40(GJA5).
PMID:15782139
Cx32 has a strong tumor-suppressive effect on a human metastatic renal cell carcinoma cell line.
Cx32-dependent inactivation of Src decreased the production of VEGF via the suppression of Stat3 activation
e_re1009( EMT  map ):
PMID:16371504
Phosphorylation of N-cadherin at Y860 by SRC decreases its ability to bind to CTNNB1
CTNNB1 dissociated from the complex with N-cadherin enters to the nucleus.
e_re1010( EMT  map ):
PMID:10593980
PMID:12123611
Phosphorylation of CTNNB1 at Y654 by SRC prevents the interaction between Cadherin and CTNNB1.
This phosphorylation therefore reduces adhesive function.


Modifications:
In compartment: Cytoplasm
  1. v-SRC*@Cytoplasm map

Participates in complexes:
In compartment: Cytoplasm

  1. GJB1:​v-SRC*@Cytoplasm map

Participates in reactions:
As Reactant or Product:

  1. GJB1@Cytoplasm map + v-SRC*@Cytoplasm map map GJB1:​v-SRC*@Cytoplasm map

  2. Src family kinases*@Cytoplasm map map v-SRC*@Cytoplasm map

As Catalyser:

  1. N-Cadherin*@Cytoplasm map map N-Cadherin*|​pho@Cytoplasm map

  2. _beta_-Catenin*@Cytoplasm map map _beta_-Catenin*|​pho@Cytoplasm map
  3. Occludin*@Cytoplasm map + ATP@Cytoplasm map map Occludin*|​pho@Cytoplasm map + ADP@Cytoplasm map
  4. GJA1@Cytoplasm map + ATP@Cytoplasm map map GJA1|​pho@Cytoplasm map + ADP@Cytoplasm map

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