SMAD3:SMAD4

Complex SMAD3:SMAD4 map


Complex composition:

  1. SMAD4 map
  2. SMAD3 map




(SMAD3|​S423_pho|​S425_pho|​hm2:​SMAD4)|​active@Nucleus

Identifiers
NAME:SMAD3:SMAD4

Maps_Modules
 EMT  map  / EMT_REGULATORS  map

References
e_re233( EMT  map ):
PMID:11792802
PMID:8893010
All mammalian R-Smads and Smad4 reside in the cytoplasm
Phosphorylated R-Smads quickly form complexes with Smad4 and possibly prior to nuclear translocation
PMID:9389648
Nuclear translocation of R-Smads is independent of Smad4
Whereas translocation of Smad4 after TGFB siglaing seems to require the presence of an activated R-Smad
e_re4( EMT  map ):
PMID:20731704
PMID:19208835
TCF8 (ZEB1) is up-regulated in endothelial cells during angiogenesis, acting as a negative regulator.
PMID:21317430
In NMuMG cells treated with TGFB1 Snail1 RNA and protein are induced 1 h after addition of the cytokine preceding Zeb1 up-regulation that requires 6–8 h.
Zeb1 gene expression is caused by increased RNA levels but also by enhanced protein stability and is markedly dependent on Snail1 because depletion of this protein prevents Zeb1 protein and RNA up-regulation
Snail1 controls Zeb1 expression at multiple levels and acts cooperatively with Twist in the ZEB1 gene transcription induction
PMID:16831886
PMID:18832382
HMGA2 directly binds to the SNAIL1 promoter and acts as a transcriptional regulator of SNAIL1 expression.
HMGA2 cooperates with SMAD3 and SMAD4 to execute a dramatic super-induction of the SNAIL1 promoter.
Same results were obtained with SNAI2, ZEB1, ZEB2 and TWIST1
HMGA2 cooperates with TGFB1 signaling to represse ID2 transcriptomal expression
e_re235( EMT  map ):
PMID:19597490
SMAD3 and SMAD4 interact and form a complex with SNAIL1
e_re1363( EMT  map ):
PMID:14976548
TGFB stimulates two kinetically coordinated waves of activation of HEY1.
Immediate-early activation of HEY1 by TGFB is mediated directly by Smad3/4 binding at two distinct sequences in the distal HEY1 promoter region and does not require Notch signalling.
The Smad-dependent, Notch-independent immediate-early activation is followed by a second wave of HEY1 activation.
This second wave is mediated by classical Notch-dependent signalling induced through indirect and delayed induction of Jag1 synthesis by TGFB

Confidence
REF=5 FUNC=5

(SMAD3|​S423_pho|​S425_pho|​hm2:​SMAD4)|​active@Cytoplasm

Identifiers
NAME:SMAD3:SMAD4

Maps_Modules
 EMT  map  / EMT_REGULATORS  map

References
e_re231( EMT  map ):
PMID:11224571
Phosphorylated Smad3 recruits Smad4 to form a heterotrimer
containing 2 Smad3 and 1 Smad4.
e_re233( EMT  map ):
PMID:11792802
PMID:8893010
All mammalian R-Smads and Smad4 reside in the cytoplasm
Phosphorylated R-Smads quickly form complexes with Smad4 and possibly prior to nuclear translocation
PMID:9389648
Nuclear translocation of R-Smads is independent of Smad4
Whereas translocation of Smad4 after TGFB siglaing seems to require the presence of an activated R-Smad

Confidence
REF=3 FUNC=5


Modifications:
Participates in complexes:
In compartment: Cytoplasm
  1. (SMAD3|​S423_pho|​S425_pho|​hm2:​SMAD4)|​active@Cytoplasm map

In compartment: Nucleus

  1. (SMAD3|​S423_pho|​S425_pho|​hm2:​SMAD4)|​active@Nucleus map

Participates in reactions:
As Reactant or Product:

  1. (SMAD3|​S423_pho|​S425_pho|​hm2:​SMAD4)|​active@Nucleus map + HMGA2@Nucleus map map HMGA2:​SMAD2_3*:​SMAD4@Nucleus map

  2. SMAD4@Cytoplasm map + SMAD3|​S423_pho|​S425_pho|​hm2|​active@Cytoplasm map map (SMAD3|​S423_pho|​S425_pho|​hm2:​SMAD4)|​active@Cytoplasm map
  3. (SMAD3|​S423_pho|​S425_pho|​hm2:​SMAD4)|​active@Cytoplasm map map (SMAD3|​S423_pho|​S425_pho|​hm2:​SMAD4)|​active@Nucleus map
  4. SNAI1@Nucleus map + (SMAD3|​S423_pho|​S425_pho|​hm2:​SMAD4)|​active@Nucleus map map (SMAD3|​S423_pho|​S425_pho|​hm2:​SMAD4:​SNAI1)|​active@Nucleus map
  5. (SMAD3|​S423_pho|​S425_pho|​hm2:​SMAD4)|​active@Nucleus map + SP1@Nucleus map map SMAD3|​S423_pho|​S425_pho|​hm2:​SMAD4:​SP1@Nucleus map

As Catalyser:

  1. gHEY1@Nucleus map map rHEY1@Nucleus map

  2. gZEB1@Nucleus map map rZEB1@Nucleus map

Leave a Reply