Occludin*:PARD6A:TGFB1:TGFBR1:TGFBR2

Complex Occludin*:PARD6A:TGFB1:TGFBR1:TGFBR2 map


Complex composition:

  1. Occludin* map
  2. TGFB1 map
  3. PARD6A map
  4. TGFBR2 map
  5. TGFBR1 map




Occludin*:​PARD6A:​TGFB1|​hm2:​TGFBR1|​hm2:​TGFBR2|​hm2@Cytoplasm

Identifiers
NAME:Occludin*:PARD6A:TGFB1:TGFBR1:TGFBR2

Maps_Modules
 EMT  map  / EMT_REGULATORS  map
 EMT  map  / CELL_CELL_ADHESIONS  map
 EMT  map  / TIGHT_JUNCTIONS  map

References
PMID:15761153
Occludin is a structural component of tight junctions that is associated with the cell polarity network.
Occludin regulates TGFBR1 localization for efficient TGFB-dependent dissolution of tight junctions during EMT
Interaction of endogenous OCLN with endogenous TGFBR1 was not modulated by TGFB
but its association with the TGFBR2 increased in a TGFB-dependent manner
PMID:15761148
TGFBR1 localizes with ZO-1 on the cellular apical aspect together with PARD6A and they are situated apically to endogenous E-cadherin
After stimulation by TGFB, TGFBR2 is redistributed to the tight junctions, where it localizes with both TGFBR1 and ZO-1.
e_re341( EMT  map ):
Active TGFBR2 recruited to TIGHT_JUNCTIONS phosphorylates PARD6A on Serine residue S345 and it also phosphorylates TGFBR1
Once phosphorylated by TGFBR2, both TGFBR1 and PARD6A become active

Confidence
REF=2 FUNC=4

Occludin*:​PARD6A|​S345_pho:​TGFB1|​hm2:​TGFBR1|​Ser_pho|​hm2:​TGFBR2|​Ser_pho|​hm2@Cytoplasm

Identifiers
NAME:Occludin*:PARD6A:TGFB1:TGFBR1:TGFBR2

Maps_Modules
 EMT  map  / EMT_REGULATORS  map
 EMT  map  / CELL_CELL_ADHESIONS  map
 EMT  map  / TIGHT_JUNCTIONS  map

References
PMID:15761148
After stimulation by TGFB, TGFBR2 is redistributed to the tight junctions, where it localizes with both TGFBR1 and ZO-1.
Active TGFBR2 recruited to TIGHT_JUNCTIONS phosphorylates PARD6A on Serine residue S345 and it also phosphorylates TGFBR1
Once phosphorylated by TGFBR2, both TGFBR1 and PARD6A become active
e_re343( EMT  map ):
PMID:21572392
SMURF1 is an homologous to E6AP C-ter (HECT)-type E3 ubiquitin ligase
SMURF1 performs a crucial role in the regulation of the BMP signalling pathway in both embryonic development and bone remodelling.
PARD6A interacts with TGFB receptors and is phsophorylated by TGFBRIII.
Phosphorylation of Par6 is required for TGFB-dependent EMT in mammary gland epithelial cells
This phosphorylation controls the interaction of PARD6A with the E3 ubiquitin ligase Smurf1.
Smurf1, in turn, targets GTPase RhoA for degradation, thereby leading to a loss of tight junctions.
PMID:22949611
Signaling molecules act directly on polarity proteins, bypassing transcription factors such as Snail and Zeb1:
TGFBRI binds to the tight junction protein Occludin and locally assembles into a complex containing Par6.
Activated TGFBRII phosphorylates Par6, which binds to Smurf1 and causes RhoA ubiquitylation and the dissolution of junctions.

Confidence
REF=2 FUNC=4


Modifications:
Participates in complexes:
In compartment: Cytoplasm
  1. Occludin*:​PARD6A:​TGFB1|​hm2:​TGFBR1|​hm2:​TGFBR2|​hm2@Cytoplasm map

  2. Occludin*:​PARD6A|​S345_pho:​TGFB1|​hm2:​TGFBR1|​Ser_pho|​hm2:​TGFBR2|​Ser_pho|​hm2@Cytoplasm map

Participates in reactions:
As Reactant or Product:

  1. Occludin*:​PARD6A:​TGFB1|​hm2:​TGFBR1|​hm2:​TGFBR2|​hm2@Cytoplasm map + ATP@Cytoplasm map map Occludin*:​PARD6A|​S345_pho:​TGFB1|​hm2:​TGFBR1|​Ser_pho|​hm2:​TGFBR2|​Ser_pho|​hm2@Cytoplasm map + ADP@Cytoplasm map

  2. Occludin*:​PARD6A:​TGFBR1|​hm2@Cytoplasm map + TGFB1|​hm2:​TGFBR1|​hm2:​TGFBR2|​hm2@Cytoplasm map map Occludin*:​PARD6A:​TGFB1|​hm2:​TGFBR1|​hm2:​TGFBR2|​hm2@Cytoplasm map
  3. Occludin*:​PARD6A|​S345_pho:​TGFB1|​hm2:​TGFBR1|​Ser_pho|​hm2:​TGFBR2|​Ser_pho|​hm2@Cytoplasm map + SMURF1@Cytoplasm map map PARD6A|​S345_pho:​SMURF1@Cytoplasm map

As Catalyser:

  1. Occludin*:​PARD6A:​TGFB1|​hm2:​TGFBR1|​hm2:​TGFBR2|​hm2@Cytoplasm map + ATP@Cytoplasm map map Occludin*:​PARD6A|​S345_pho:​TGFB1|​hm2:​TGFBR1|​Ser_pho|​hm2:​TGFBR2|​Ser_pho|​hm2@Cytoplasm map + ADP@Cytoplasm map

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