Occludin*

Protein Occludin* map

Identifiers
occludin
HUGO:OCLN HGNC:8104 ENTREZ:100506658 UNIPROT:Q16625 GENECARDS:OCLN REACTOME:60846 KEGG:100506658 ATLASONC:GC_OCLN WIKI:OCLN

Maps_Modules
 EMT  map  / EMT_REGULATORS  map
 EMT  map  / CELL_CELL_ADHESIONS  map
 EMT  map  / TIGHT_JUNCTIONS  map

References
PMID:15761153
Occludin is a structural component of tight junctions that is associated with the cell polarity network.
Occludin regulates TGFBR1 localization for efficient TGFB-dependent dissolution of tight junctions during EMT
Interaction of endogenous OCLN with endogenous TGFBR1 was not modulated by TGFB
but its association with the TGFBR2 increased in a TGFB-dependent manner
PMID:15761148
TGFBR1 localizes with ZO-1 on the cellular apical aspect together with PARD6A and they are situated apically to endogenous E-cadherin
After stimulation by TGFB, TGFBR2 is redistributed to the tight junctions, where it localizes with both TGFBR1 and ZO-1.
PMID:22315516
-Occludin is phosphorylated at S340 by PKC
-Occludin is phosphorylated at T383 and S508 by ROCK
-Occludin is phosphorylated at Y398, Y402 and Y 474 by SRC
-Occludin is phosphorylated at T400, T404 and S408 by CK2
-Occludin is phosphorylated at S403 and S404 by PKC-N
-Occludin is phosphorylated at T424 and T438 by PKC-E
-Occludin is phosphorylated at S490 by VEGF

Occludin*@Cytoplasm

Maps_Modules
 EMT  map  / EMT_REGULATORS  map
 EMT  map  / CELL_CELL_ADHESIONS  map
 EMT  map  / TIGHT_JUNCTIONS  map

References
e_re340( EMT  map ):
PMID:15761153
Occludin is a structural component of tight junctions that is associated with the cell polarity network.
Occludin regulates TGFBR1 localization for efficient TGFB-dependent dissolution of tight junctions during EMT
Interaction of endogenous OCLN with endogenous TGFBR1 was not modulated by TGFB
but its association with the TGFBR2 increased in a TGFB-dependent manner
PMID:15761148
TGFBR1 localizes with ZO-1 on the cellular apical aspect together with PARD6A and they are situated apically to endogenous E-cadherin
After stimulation by TGFB, TGFBR2 is redistributed to the tight junctions, where it localizes with both TGFBR1 and ZO-1.
e_re474( EMT  map ):
PMID:22315516
-Occludin is phosphorylated at S340 by PKC
-Occludin is phosphorylated at T383 and S508 by ROCK
-Occludin is phosphorylated at Y398, Y402 and Y474 by SRC
-Occludin is phosphorylated at T400, T404 and S408 by CK2
-Occludin is phosphorylated at S403 and S404 by PKC-N
-Occludin is phosphorylated at T424 and T438 by PKC-E
-Occludin is phosphorylated at S490 by VEGF
PMID:19017651
PMID:12604349
In mouse, phosphorylation of Occludin by SRC (at Y398, Y402) reduces TIGHT_JUNCTIONS assembly
In mouse, phosphorylation of Occludin by SRC (at Y398, Y402) reduces interaction between Occludin and ZO1,2,3
PMID:20152177
In mouse, phosphorylation of Occludin by SRC (at Y474) increases interaction between Occludin and PI3K
In mouse, phosphorylation of Occludin by SRC (at Y474) increases wound healing and migration
PMID:12547828
Inactive Src delays the oxidative stress-induced disruption of tight junction and accelerates calcium- induced assembly of tight junction in Caco-2 cells.
This demonstrates that oxidative stress-induced disruption of tight junction is mediated by the activation of Src.
e_re516( EMT  map ):
PMID:15383321
MTDH is so-called LYRIC
LYRIC colocalizes with ZO1 and Occludin in polarized epithelial cells.
This protein is most likely not involved in the TJ formation as a structural component.
However, it is required for the maturation of the TJ complex
e_re518( EMT  map ):
PMID:22671595
Caveolin-1 is an endocytic scaffolding protein
It binds independently to claudin-2 and occludin (co-immunoprecipitation assays).
The finding that caveolin-1 interacts with claudin-2 and occludin, but notwith claudin-4 or ZO-1, suggests a potential mechanism for selective retrieval of tight junction components.
e_re651( EMT  map ):
PMID:11978007
Cx32 Formation and / or Cx32-Mediated Intercellular Communication Induces Expression and Function of Tight Junctions in Hepatocytic Cell Line
PMID:10581193
Induction of tight junctions in human connexin 32-transfected mouse hepatocytes: connexin 32 interacts with occludin.
PMID:17568974
Connexins induce and maintain tight junctions in epithelial cells.

Occludin*@Extracellular space

Maps_Modules
 EMT  map  / EMT_REGULATORS  map
 EMT  map  / CELL_CELL_ADHESIONS  map
 EMT  map  / TIGHT_JUNCTIONS  map

Occludin*|​pho@Cytoplasm

Maps_Modules
 EMT  map  / EMT_REGULATORS  map
 EMT  map  / CELL_CELL_ADHESIONS  map
 EMT  map  / TIGHT_JUNCTIONS  map

References
e_re474( EMT  map ):
PMID:22315516
-Occludin is phosphorylated at S340 by PKC
-Occludin is phosphorylated at T383 and S508 by ROCK
-Occludin is phosphorylated at Y398, Y402 and Y474 by SRC
-Occludin is phosphorylated at T400, T404 and S408 by CK2
-Occludin is phosphorylated at S403 and S404 by PKC-N
-Occludin is phosphorylated at T424 and T438 by PKC-E
-Occludin is phosphorylated at S490 by VEGF
PMID:19017651
PMID:12604349
In mouse, phosphorylation of Occludin by SRC (at Y398, Y402) reduces TIGHT_JUNCTIONS assembly
In mouse, phosphorylation of Occludin by SRC (at Y398, Y402) reduces interaction between Occludin and ZO1,2,3
PMID:20152177
In mouse, phosphorylation of Occludin by SRC (at Y474) increases interaction between Occludin and PI3K
In mouse, phosphorylation of Occludin by SRC (at Y474) increases wound healing and migration
PMID:12547828
Inactive Src delays the oxidative stress-induced disruption of tight junction and accelerates calcium- induced assembly of tight junction in Caco-2 cells.
This demonstrates that oxidative stress-induced disruption of tight junction is mediated by the activation of Src.


Modifications:
In compartment: Cytoplasm
  1. Occludin*@Cytoplasm map

  2. Occludin*|​pho@Cytoplasm map

In compartment: Extracellular space

  1. Occludin*@Extracellular space map

  2. Occludin*|​hm2@Extracellular space map

Participates in complexes:
In compartment: Cytoplasm

  1. GJB1:​Occludin*@Cytoplasm map

  2. LYRIC*:​Occludin*@Cytoplasm map
  3. Nectin*:​Occludin*@Cytoplasm map
  4. Cingulin*:​Occludin*@Cytoplasm map
  5. Claudin1*:​Occludin*@Cytoplasm map
  6. Claudin2*:​Occludin*@Cytoplasm map
  7. Caveolin1*:​Occludin*@Cytoplasm map
  8. Actin cytoskeletal*:​Occludin*@Cytoplasm map
  9. Occludin*:​PARD6A:​TGFBR1|​hm2@Cytoplasm map
  10. Occludin*:​PARD6A:​TGFB1|​hm2:​TGFBR1|​hm2:​TGFBR2|​hm2@Cytoplasm map
  11. Occludin*:​PARD6A|​S345_pho:​TGFB1|​hm2:​TGFBR1|​Ser_pho|​hm2:​TGFBR2|​Ser_pho|​hm2@Cytoplasm map

Participates in reactions:
As Reactant or Product:

  1. PARD6A@Cytoplasm map + TGFBR1|​hm2@Cytoplasm map + Occludin*@Cytoplasm map map Occludin*:​PARD6A:​TGFBR1|​hm2@Cytoplasm map

  2. Occludin*:​PARD6A:​TGFB1|​hm2:​TGFBR1|​hm2:​TGFBR2|​hm2@Cytoplasm map + ATP@Cytoplasm map map Occludin*:​PARD6A|​S345_pho:​TGFB1|​hm2:​TGFBR1|​Ser_pho|​hm2:​TGFBR2|​Ser_pho|​hm2@Cytoplasm map + ADP@Cytoplasm map
  3. Occludin*:​PARD6A:​TGFBR1|​hm2@Cytoplasm map + TGFB1|​hm2:​TGFBR1|​hm2:​TGFBR2|​hm2@Cytoplasm map map Occludin*:​PARD6A:​TGFB1|​hm2:​TGFBR1|​hm2:​TGFBR2|​hm2@Cytoplasm map
  4. Occludin*:​PARD6A|​S345_pho:​TGFB1|​hm2:​TGFBR1|​Ser_pho|​hm2:​TGFBR2|​Ser_pho|​hm2@Cytoplasm map + SMURF1@Cytoplasm map map PARD6A|​S345_pho:​SMURF1@Cytoplasm map
  5. rOccludin*@Nucleus map map Occludin*@Cytoplasm map
  6. Occludin*@Cytoplasm map + ATP@Cytoplasm map map Occludin*|​pho@Cytoplasm map + ADP@Cytoplasm map
  7. Occludin*@Extracellular space map + Occludin*@Cytoplasm map map Occludin*|​hm2@Extracellular space map
  8. Occludin*@Cytoplasm map + Claudin1*@Cytoplasm map map Claudin1*:​Occludin*@Cytoplasm map
  9. Occludin*@Cytoplasm map + Claudin2*@Cytoplasm map map Claudin2*:​Occludin*@Cytoplasm map
  10. Occludin*@Cytoplasm map + Cingulin*@Cytoplasm map map Cingulin*:​Occludin*@Cytoplasm map
  11. Occludin*@Cytoplasm map + Nectin*@Cytoplasm map map Nectin*:​Occludin*@Cytoplasm map
  12. LYRIC*@Cytoplasm map + Occludin*@Cytoplasm map map LYRIC*:​Occludin*@Cytoplasm map
  13. Occludin*@Cytoplasm map + Actin cytoskeletal*@Cytoplasm map map Actin cytoskeletal*:​Occludin*@Cytoplasm map
  14. Occludin*@Cytoplasm map + Caveolin1*@Cytoplasm map map Caveolin1*:​Occludin*@Cytoplasm map
  15. Occludin_Claudin1_2_4_5_7_8_16*@Cytoplasm map map Occludin*@Cytoplasm map
  16. Occludin*|​hm2@Extracellular space map map Tight junctions@Extracellular space map
  17. Claudin1*:​Occludin*@Cytoplasm map map Tight junctions@Extracellular space map
  18. Claudin2*:​Occludin*@Cytoplasm map map Tight junctions@Extracellular space map
  19. Actin cytoskeletal*:​Occludin*@Cytoplasm map map Tight junctions@Extracellular space map
  20. Occludin*@Cytoplasm map + GJB1@Cytoplasm map map GJB1:​Occludin*@Cytoplasm map
  21. GJB1:​Occludin*@Cytoplasm map map Tight junctions@Extracellular space map

As Catalyser:

  1. Occludin*:​PARD6A:​TGFB1|​hm2:​TGFBR1|​hm2:​TGFBR2|​hm2@Cytoplasm map + ATP@Cytoplasm map map Occludin*:​PARD6A|​S345_pho:​TGFB1|​hm2:​TGFBR1|​Ser_pho|​hm2:​TGFBR2|​Ser_pho|​hm2@Cytoplasm map + ADP@Cytoplasm map

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