HIF1A

Protein HIF1A map

Identifiers
hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
HUGO:HIF1A HGNC:4910 ENTREZ:3091 UNIPROT:Q16665 GENECARDS:HIF1A REACTOME:56516 KEGG:3091 ATLASONC:GC_HIF1A WIKI:HIF1A

Maps_Modules
 EMT  map  / EMT_REGULATORS  map
 Apoptosis  map  / APOPTOSIS_GENES  map
 Apoptosis  map  / HIF1  map

References
The gene HIF1A gives rise to 3 different isoforms: HIF1_alpha_, HIF2_alpha_ and HIF3_alpha

HIF1A@Cytoplasm

References
e_re1257( EMT  map ):
PMID:10833514
Hypoxia increases mitochondrial ROS generation at Complex III, which causes accumulation of HIF-1alpha protein
Mitochondria-derived ROS are both required and sufficient to initiate HIF-1 alpha stabilization during hypoxia.

HIF1A|​ubi@Nucleus

References
a_re398:( Apoptosis  map ) PMID:18838541

HIF1A|​ubi|​P564_hyd@Cytoplasm

References
a_re386( Apoptosis  map ):
PMID:10973499
in vitro reconstitution of the E3-ligase activity, with VHL, elonginB/C, RBX1,CUL2
PMID:18985005
in COS monkey kidney cells and Caki-I human renal carcinoma cells, inhibition of VHL-mediated HIF1A ubiquitinylation and degradation by ectopically expressed constitutively active STAT3

HIF1A|​P564_hyd@Cytoplasm

References
a_re387( Apoptosis  map ):
PMID:12351678
identification of PHD2 as a HIF1A prolyl hydroxylase
PMID:15652751
inhibition of HIF1A prolyl hydroxylation by succinate and fumarate
PMID:15721254
in 293 cells, OS9 interacts with HIF1 and PHD and promotes HIF1A prolylhydroxylation and degradation
a_re386( Apoptosis  map ):
PMID:10973499
in vitro reconstitution of the E3-ligase activity, with VHL, elonginB/C, RBX1,CUL2
PMID:18985005
in COS monkey kidney cells and Caki-I human renal carcinoma cells, inhibition of VHL-mediated HIF1A ubiquitinylation and degradation by ectopically expressed constitutively active STAT3

HIF1A@Nucleus

References
a_re401:( Apoptosis  map ) reactionType:is.a
a_re98( Apoptosis  map ):
PMID:18056423
p53 mediated upregulation of IGFBP1
in mouse liver
PMID:9707622
in HepG2, by HIF1
PMID:10102273
in 293T, by FOXO3, EMSA
a_re126( Apoptosis  map ):
PMID:14699081
in Saos-2, direct transactivation of NOXA by HIF1A during hypoxia, role of NOXA in hypoxia-induced cell death
PMID:16888645
in SH-EP, in NB15 neuroblastoma cell lines expressing a FOXO3-ER fusion, upon 4OHT. PMAIP knock down protects from apoptosis
PMID:12676992
in MEF by CTBP1 and CTBP2 (RT-PCR, double knocked out)
PMID:14684737
in NIH3T3, by E2F1
a_re130( Apoptosis  map ):
PMID:12402042
in mouse splenic red pulp and colonic epithelium, upon irradiation
in human, the p53 binding element is conserved
PMID:15024076
in HT29 and CHO, HIF1-dependent hypoxia-induced repression of BID expression
in SW480 upon hypoxia, interaction between HIF1 and BID promoter, by EMSA
PMID:15240700
in mouse T cells, by E2F1
a_re158( Apoptosis  map ):
Reaction (id=re340, name=; 10 01 04.xml)
PMID:11557773
by HIF1
PMID:14672622
in liver tumors by HIF1
PMID:16782875
by MLX:MLXIP
a_re160( Apoptosis  map ):
PMID:11744734
in renal carcinoma 786-0 cells (VHL deficient), in HIF1 KO MEFs
PMID:17143338
in pancreatic and gastric cancer cell lines (correlation HIF stabilisation and gene expression)
a_re162( Apoptosis  map ):
PMID:16517405
HIF
PMID:16517406
PMID:17785433
by MYC
a_re164( Apoptosis  map ):
PMID:17418790
in Hela cells, in RCC4 renal carcinoma human cells, in mouse
a_re170( Apoptosis  map ):
PMID:8955077
in HepG3
PMID:8089148
in Hela and HepG3
a_re172( Apoptosis  map ):
PMID:16126909
in human breast carcinoma BT-549 cells, by HIF1
PMID:10823814
in rat1a rat fibroblasts upon c-myc overexpression
a_re174( Apoptosis  map ):
PMID:7493931
in HepG2 by HIF1 (EMSA, reporter plasmids)
PMID:9525945
in chinese hamster ovary cells by HIF1
PMID:15059920
in Saos2, in human embryonal rhabdomyosarcoma derived RD (ATCC no. CCL-136), in murine skeletal muscle-derived C2C12 myoblasts (ATCC no. CRL-1772), by TP53 (luciferase reporter)
a_re176( Apoptosis  map ):
PMID:15379897
in CCF-STTG1 human glial cells (EMSA + supershift with HRE derived from the PKM2 promoter)
a_re178( Apoptosis  map ):
PMID:14762718
in MEFs upon hypoxia, not in HIF1 KO cells
PMID:15199147
MYC
a_re180( Apoptosis  map ):
PMID:16585195
in Hela cells, BNIP3 expression upon hypoxia is impaired by siRNA against HIF1
PMID:18054315
PMID:11559532
a_re182( Apoptosis  map ):
in Hela cells, PGK1 expression upon hypoxia is impaired by siRNA against HIF1
PMID:8702901
in mouse hepatoma cell line Hepa1c1c7, but not in an ARNT defective conterpart
a_re184( Apoptosis  map ):
in Hela cells, CA9 expression upon hypoxia is impaired by siRNA against HIF1
a_re186:( Apoptosis  map ) PMID:18718909
a_re189( Apoptosis  map ):
PMID:7508926
in murine RAW 264.7 macrophage cell line, by NFKB
PMID:7500013
in murine macrophage derived from C57BL/6 mice and transformed with J2 (v-raf1v-myc) recombinant retrovirus, by HIF1
PMID:9486205
in rat lung epithelia, by hypoxia
in bovine pulmonary artery endothelial cells, HIF1 regulates the murine iNOS promoter (supershifted EMSA + gene reporter)
a_re190( Apoptosis  map ):
PMID:19211554
in PC-3 human prostate cancer cell line by HIF, transactivation of the promoter (ChIP, EMSA, reporter gene assay)
PMID:10435632
in murine immortalized bone marrow-derived pro-B-cell BA/F3, by STAT5 upon IL3
PMID:10428780 AND PMID:10428030
in murine erythroleukemia HCD-57 cells, by STAT5 upon EPO (HGNC:3415) treatment
PMID:11756417
in UT-7/TPO human TPO-dependent megakaryocytic cell line, by STAT5 and NF-kappaB, upon TPO
a_re192( Apoptosis  map ):
PMID:18158893
in MEFs, in MCF7
a_re194( Apoptosis  map ):
PMID:15545625
PMID:11884613
a_re196( Apoptosis  map ):
prototypical HIF1 target
PMID:15369676
a_re199:( Apoptosis  map ) PMID:20028863
a_re876:( Apoptosis  map ) PMID:15312748
a_re992( Apoptosis  map ):
PMID:12743597
in 786-O human carcinoma cell line


Modifications:
In compartment: Cytoplasm
  1. HIF1A@Cytoplasm map

  2. HIF1A|​P564_hyd@Cytoplasm map
  3. HIF1A|​ubi|​P564_hyd@Cytoplasm map

In compartment: Nucleus

  1. HIF1A@Nucleus map

  2. HIF1A|​ubi@Nucleus map

Participates in complexes:
In compartment: Nucleus

  1. CITED2:​HIF1A@Nucleus map

  2. HIF1A:​p300*|​unk@Nucleus map

Participates in reactions:
As Reactant or Product:

  1. rHIF1A@Nucleus map map HIF1A@Cytoplasm map

  2. HIF1A|​P564_hyd@Cytoplasm map map HIF1A|​ubi|​P564_hyd@Cytoplasm map
  3. HIF1A@Cytoplasm map + O_sub_2_endsub_@Cytoplasm map + 2-oxoglutarate@Cytoplasm map map HIF1A|​P564_hyd@Cytoplasm map + succinate@Cytoplasm map + CO_sub_2_endsub_@Cytoplasm map
  4. HIF1A|​ubi|​P564_hyd@Cytoplasm map map degraded
  5. p300*|​unk@Nucleus map + a_s3082 map HIF1A:​p300*|​unk@Nucleus map
  6. HIF1A@Nucleus map map HIF1A|​ubi@Nucleus map
  7. HIF1A|​ubi@Nucleus map map degraded
  8. HIF1A@Nucleus map + HIF1B*@Nucleus map map a_s3082
  9. HIF1A:​p300*|​unk@Nucleus map map HIF1A@Nucleus map
  10. HIF1A@Cytoplasm map map HIF1A@Nucleus map
  11. a_s3082 + CITED2@Nucleus map map CITED2:​HIF1A@Nucleus map
  12. HIF_alpha_*@Cytoplasm map map HIF1A@Cytoplasm map
  13. ROS@Mitochondria map map HIF1A@Cytoplasm map

As Catalyser:

  1. gPMAIP1@Nucleus map map rPMAIP1@Nucleus map

  2. gBID@Nucleus map map rBID@Nucleus map
  3. gHK2@Nucleus map map rHK2@Nucleus map
  4. gPFKFB3@Nucleus map map rPFKFB3@Nucleus map
  5. gPDK1@Nucleus map map rPDK1@Nucleus map
  6. gLONP1@Nucleus map map rLONP1@Nucleus map
  7. gCOX4I2@Nucleus map map rCOX4I2@Nucleus map
  8. gPFKFB4@Nucleus map map rPFKFB4@Nucleus map
  9. gALDOA@Nucleus map map rALDOA@Nucleus map
  10. gGPI@Nucleus map map rGPI@Nucleus map
  11. gSLC2A1@Nucleus map map rSLC2A1@Nucleus map
  12. gPKM2@Nucleus map map rPKM2@Nucleus map
  13. gTPI1@Nucleus map map rTPI1@Nucleus map
  14. gBNIP3@Nucleus map map rBNIP3@Nucleus map
  15. gPGK1@Nucleus map map rPGK1@Nucleus map
  16. gCA9@Nucleus map map rCA9@Nucleus map
  17. gPDK3@Nucleus map map rPDK3@Nucleus map
  18. gNOS2@Nucleus map map rNOS2@Nucleus map
  19. gBCL2-XL*@Nucleus map map rBCL2-XL*@Nucleus map
  20. gFOXO3@Nucleus map map rFOXO3@Nucleus map
  21. gDDIT4@Nucleus map map rDDIT4@Nucleus map
  22. gVEGFA@Nucleus map map rVEGFA@Nucleus map
  23. gEGLN1@Nucleus map map rEGLN1@Nucleus map
  24. gEGLN3@Nucleus map map rEGLN2@Nucleus map
  25. gFECH@Nucleus map map rFECH@Nucleus map
  26. gIGFBP1@Nucleus map map rIGFBP1@Nucleus map
  27. gCyclinD1*@Nucleus map map rCyclinD1*@Nucleus map

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