H2AFX

Protein H2AFX map

Identifiers
H2A histone family, member X
HUGO:H2AFX HGNC:4739 ENTREZ:3014 UNIPROT:P16104 GENECARDS:H2AFX REACTOME:56152 KEGG:3014 ATLASONC:H2AFXID40783ch11q23 WIKI:H2AFX

Maps_Modules
 Apoptosis  map  / MITOCH_METABOLISM  map
 DNA repair  map  / S_PHASE_CHECKPOINT  map
 DNA repair  map  / G2_M_CHECKPOINT  map
 Survival  map  / WNT_CANONICAL  map

References
synonym:H2AX
PMID:9488723, PMID:19772495, PMID:17303408
For S-phase checkpoint
PMID:15459660, PMID:12607003

H2AFX|​S139_pho@Site of DNA damage

References
a_re1488( Apoptosis  map ):
influenceDelete: re to reactants
PMID:9488723
PMID:11571274
a_re1479( Apoptosis  map ):
PMID:16377563
PMID:16049003
a_re1489( Apoptosis  map ):
PMID:20118229
by PPM1D
PMID:20101220

H2AFX|​Y142_pho@Nucleus

References
a_re1490( Apoptosis  map ):
influenceDelete: reactants to re
PMID:19092802
by WICH complex (subunit identification by mass spectrometry: WSTF and SNF2H)
PMID:19234442
a_re1494( Apoptosis  map ):
PMID:16818236
upon UVA irradiation (no DSB), phosphorylation of H2AX S139 by JNK
effect of Y142 phosphorylation not assessed
interaction between JNK1 and H2AX Y142_pho dependent on FE65 (proposed FE65 scaffolding JNK to Y142_pho)
they propose H2AX Y142 phosphorylation as a switch toward apoptosis.
Even if not fully documented, the information from these 2 papers is merged into this representation where doubly phosphorylated H2AX cooperated with CAD for DNA fragmentation

H2AFX@Nucleus

References
a_re1489( Apoptosis  map ):
PMID:20118229
by PPM1D
PMID:20101220
a_re1495:( Apoptosis  map ) PMID:19234442
a_re1488( Apoptosis  map ):
influenceDelete: re to reactants
PMID:9488723
PMID:11571274
a_re1490( Apoptosis  map ):
influenceDelete: reactants to re
PMID:19092802
by WICH complex (subunit identification by mass spectrometry: WSTF and SNF2H)
PMID:19234442

H2AFX|​S139_pho|​Y142_pho@Nucleus

References
a_re1494( Apoptosis  map ):
PMID:16818236
upon UVA irradiation (no DSB), phosphorylation of H2AX S139 by JNK
effect of Y142 phosphorylation not assessed
PMID:19234442
interaction between JNK1 and H2AX Y142_pho dependent on FE65 (proposed FE65 scaffolding JNK to Y142_pho)
they propose H2AX Y142 phosphorylation as a switch toward apoptosis.
Even if not fully documented, the information from these 2 papers is merged into this representation where doubly phosphorylated H2AX cooperated with CAD for DNA fragmentation
a_re1044( Apoptosis  map ):
PMID:12198487
by AIFM1
PMID:14716299
in vitro, by AIF+CypA
PMID:11452314
in DFFB KO MEFs by ENDOG

H2AFX|​pho|​ubi@default

References
d_re147( DNA repair  map ):
PMID:18006705
RNF8:MDC1 complex recruit HR-related proteins by this shifting from C_NHEJ to HR:
PMID:18001825
PMID:17303408
d_re149:( DNA repair  map ) PMID:17303408, PMID:16377563
d_re166( DNA repair  map ):
Fanconi recognition complex as FA core complex recruits NER, HR, TLS proteins that collectively repair the ICL. If Fanconi proteins participating in the recognition are dysactivated, there is no any other mechanism to recognize ILSs, therefore, even if NER, HR and TLS are correct, they will not perform the repair without core Fanconi proteins.
PMID:19686080

H2AFX|​pho@default

References
d_re90( DNA repair  map ):
PMID:9488723, PMID:19772495
PMID:17303408
d_re92:( DNA repair  map ) PMID:16310392
d_re147( DNA repair  map ):
PMID:18006705
RNF8:MDC1 complex recruit HR-related proteins by this shifting from C_NHEJ to HR:
PMID:18001825

H2AFX@default

References
d_re92:( DNA repair  map ) PMID:16310392
d_re90( DNA repair  map ):
PMID:9488723, PMID:19772495
PMID:17303408


Modifications:
In compartment: Nucleus
  1. H2AFX@Nucleus map

  2. H2AFX|​Y142_pho@Nucleus map
  3. H2AFX|​S139_pho|​Y142_pho@Nucleus map

In compartment: Site of DNA damage

  1. H2AFX|​S139_pho@Site of DNA damage map

In compartment: default

  1. H2AFX@default map

  2. H2AFX|​pho@default map
  3. H2AFX|​pho|​ubi@default map

Participates in complexes:
In compartment: Nucleus

  1. Actin*:​BAF53A*:​BRD8:​DMAP1:​EAF6*:​GAS41*:​H2AFX:​H2AFZ:​H2B*:​ING3:​MRG15*:​MRGBP:​Pontin*:​Reptin*:​TRRAP:​YL1*:​p400*@Nucleus map

In compartment: default

  1. H2AFX:​PARP1@default map

  2. H2AFX|​pho|​ubi:​MDC1|​pho@default map
  3. ATM|​pho|​S367_pho|​S1893_pho:​H2AFX|​pho|​ubi:​MDC1|​pho:​MRE11*:​NBS1*|​S343_pho:​RAD50@default map
  4. BRCA1:​BRCA2:​FANCD2|​pho:​FANCI|​pho:​H2AFX|​pho|​ubi:​NBS1*|​S343_pho:​PCNA:​RAD51:​TIP60*@default map
  5. BRCA1:​BRCA2:​FANCD2|​K561_ubi|​pho:​FANCI|​K523_ubi|​pho:​H2AFX|​pho|​ubi:​NBS1*|​S343_pho:​PCNA:​RAD51:​TIP60*@default map

Participates in reactions:
As Reactant or Product:

  1. H2AFX@Nucleus map map H2AFX|​S139_pho@Site of DNA damage map

  2. H2AFX|​S139_pho@Site of DNA damage map map H2AFX@Nucleus map
  3. H2AFX@Nucleus map map H2AFX|​Y142_pho@Nucleus map
  4. H2AFX|​Y142_pho@Nucleus map map H2AFX|​S139_pho|​Y142_pho@Nucleus map
  5. H2AFX|​Y142_pho@Nucleus map map H2AFX@Nucleus map
  6. H2AFX|​pho@default map map H2AFX|​pho|​ubi@default map
  7. H2AFX|​pho|​ubi@default map + MDC1|​pho@default map map H2AFX|​pho|​ubi:​MDC1|​pho@default map
  8. FANCD2|​K561_ubi|​pho:​FANCI|​K523_ubi|​pho@default map + BRCA1|​S988_pho|​S1387_pho|​S1423_pho|​S1497_pho|​S1524_pho|​active@default map + BRCA2@default map + NBS1*|​S343_pho@default map + RAD51@default map + PCNA@default map + H2AFX|​pho|​ubi@default map + TIP60*@default map map BRCA1:​BRCA2:​FANCD2|​K561_ubi|​pho:​FANCI|​K523_ubi|​pho:​H2AFX|​pho|​ubi:​NBS1*|​S343_pho:​PCNA:​RAD51:​TIP60*@default map
  9. BRCA1:​BRCA2:​FANCD2|​K561_ubi|​pho:​FANCI|​K523_ubi|​pho:​H2AFX|​pho|​ubi:​NBS1*|​S343_pho:​PCNA:​RAD51:​TIP60*@default map map BRCA1:​BRCA2:​FANCD2|​pho:​FANCI|​pho:​H2AFX|​pho|​ubi:​NBS1*|​S343_pho:​PCNA:​RAD51:​TIP60*@default map
  10. H2AFX@default map + PARP1|​PolyADPribose_unk|​pho@Nucleus map map H2AFX:​PARP1@default map
  11. H2AFX@default map map H2AFX|​pho@default map
  12. H2AFX|​pho@default map map H2AFX@default map
  13. ATM|​pho|​S367_pho|​S1893_pho:​MRE11*:​NBS1*|​S343_pho:​RAD50@default map + H2AFX|​pho|​ubi:​MDC1|​pho@default map map ATM|​pho|​S367_pho|​S1893_pho:​H2AFX|​pho|​ubi:​MDC1|​pho:​MRE11*:​NBS1*|​S343_pho:​RAD50@default map

As Catalyser:

  1. DNA~integrity@Nucleus map map DNA~fragmentation@Nucleus map

  2. MDC1|​Nter-SDT-motifs_pho@Nucleus map map MDC1|​Nter-SDT-motifs_pho@Site of DNA damage map
  3. gHR_DNA_st1*@Nucleus map map gHR_DNA_st2*@Nucleus map
  4. G1 phase@default map map S phase@default map
  5. S phase@default map map G2 phase@default map
  6. G2 phase@default map map M phase@default map
  7. gHR_DNA_st2*@Nucleus map map gHR_DNA_st3*@Nucleus map
  8. gFanconi_DNA_st4*@Nucleus map map gHR_DNA_st2*@Nucleus map
  9. ATM@default map map ATM|​pho|​S367_pho|​S1893_pho|​active@default map
  10. CHEK2@default map map CHEK2|​pho|​active@default map
  11. Ku70*@default map + Ku80*@default map map Ku70*:​Ku80*@Nucleus map
  12. DNA-PK*@default map map DNA-PK*|​pho@default map

Leave a Reply