Autophosphorylation of PTK2:
Autophosphorylation of PTK2 at Y397 creates binding site for CSK, a member of Scr family kinases.
In response to integrin engagement with the ECM, FAK is autophosphorylated predominantly on Y397
This Y397 is the consensus binding site for the SH2 domain of c-Src (CSK)
Interaction of CSK with FAK leads to phosphorylation of FAK on other tyrosine residues including Y407, 576, 577, 861, 925/
These phosphorylation events promote PTK2 to its maximal catalytic activity.
Upon autophosphorylation of FAK, a signaling complex of FAK, CSK binds to and can phosphorylate various adaptor proteins such as p130Cas and paxillin.