Reaction heterodimer_association e_re385
RAF1|S259_pho|S621_pho|S_pho|closed:YWHAB@Cytoplasm + GTP:HRAS@Cytoplasm → GTP:HRAS:RAF1|S259_pho|S621_pho|S_pho|closed@Cytoplasm + YWHAB@Cytoplasm
No reaction regulators
Activated HRAS (GTP bound forn) is associated with the plasma membrane.
Inactive RAF1 is associated in the cytoplasm with YWHAB via S259 phosphorylation site and also binding site.
RAF1 has a RAS-binding Cysteine-rich domain (CRD) and an additional RAS-binding domain (RBD).
RAF1 binds activated HRAS via the RBD. This binding displaces YWHAB from Ser259 and unmasks the CRD.