CDC42 promotes changes in actin cytoskeleton by several pathways.
CDC42 activates PAK1,2,3,4 and CDC42BPA (MRCKalpha) which phosphorylate LIMK1,2.
LIMK1,2 subsquently phosphorylate and inhibit Cofilin
Destrin as actin depolymerizing factor, once phosphorylated by LIMK1,2 is inactive and thus this phosphorylation leads to actin polymerization and stimulation of filopodia and stress fibers formation.
e_re844( EMT ):
CDC42SE1 (SPEC1) binds to CDC42 and supresses CDC42-induced JNK activation and cytoskeleton remodeling.
RhoGDI (so-called ARHGDIA) and GDIG4 (so-called ARHGDIB) are two known GDIs for the Rho-subfamily of GTPases.
ARHGDIG is about 50 percent identical to ARHGDIA and ARHGDIB. ARHGFIG binds to CDC42 and RhoA with less affinity compared with ARHGDIA and does not bind with Rac1, Rac2, or Ras.
ARHGDIG functions as a GDI for CDC42 but with about 20 times less efficiency than ARHGDIA.
ARHGDIA,B,G are CDC42_GDIs
e_re840( EMT ):
Spatially distinct binding of Cdc42 to PAK1 and N-WASP
e_re855( EMT ):
PARD6 contains a PDZ domain and a Cdc42/Rac interactive binding (CRIB) motif
PARD6 interacts with Rac1 and Cdc42 in a GTP-dependent manner
PARD6 binds to Cdc42/Rac1 GTPases
PARD6 is a binding partner for the Rho GTPases Cdc42 and Rac1
e_re858( EMT ):
CDC42 induces actin cytoskeleton changes by activating WASP and N-WASP.
WASP and N-WASP binds to ARP2/3 and this lieads to actin cytoskeletal polymerization and filopodia formation.
CDC42 initiates WASP/Arp2_3 nucleation of actin.
e_re864( EMT ):
CDC42 promotes cytoskeleton remodeling by binding to CDC42EPs (CDC42EP2 and CDC42EP3)
e_re872( EMT ):
CDC42 can activate JNL pathway aslo by binding and stimulation of MAP3K11 (MLK3).
Participates in complexes:
In compartment: Cytoplasm
Participates in reactions:
As Reactant or Product:
- CDC42:GDP@Cytoplasm → CDC42:GTP@Cytoplasm
- CDC42:GTP@Cytoplasm → CDC42:GDP@Cytoplasm
- PAR6*@Cytoplasm + CDC42:GTP@Cytoplasm → CDC42:GTP:PAR6*@Cytoplasm
- CDC42:GTP@Cytoplasm + CDC42EP*@Cytoplasm → CDC42:CDC42EP*@Cytoplasm