Protein Caspase9*

Identifiers
caspase 9, apoptosis-related cysteine peptidase
HUGO:CASP9 HGNC:1511 ENTREZ:842 UNIPROT:P55211 GENECARDS:CASP9 REACTOME:57034 KEGG:842 ATLASONC:CASP9ID423ch1p36 WIKI:CASP9
“caspase 9 apoptosis-related cysteine protease”

Maps_Modules
 Apoptosis   / CASPASES 
 Survival   / HEDGEHOG 
 Survival   / MAPK 
 Survival   / PI3K_AKT_MTOR 

References
PMID:19788417
PMID:16977332
Caspase9 is phosphorylated on Thr125 in a MEK1/2-dependent manner in vivo. Furthermore caspase9 is efficiently phosphorylated on Thr125 by ERK in vitro suggesting that it is targeted directly by ERK in vivo.
This is one of the ways ERK plays its anti-apoptotic role.
PMID:12792650

Caspase9*@Mitochondrial intermembrane space

References
a_re5( Apoptosis  ):
hierarchical:post-MOMP
PMID:9892620
PMID:10318956
PMID:12611882
a_re103( Apoptosis  ):
PMID:16857965
identification by Y2H
co-IP
in vitro, HAX1 prevents the processing of CASP9
in rat cardiac myocytes, HAX1 prevents the processing of CASP9 and attenuates apoptosis induced by hypoxia/reperfusion (ectopic expression, RNA interfernce)
interaction takes place within mitochondria (data not shown)
a_re104( Apoptosis  ):
PMID:14747474
in U937

Caspase9*@Cytoplasm

References
a_re5( Apoptosis  ):
hierarchical:post-MOMP
PMID:9892620
PMID:10318956
PMID:12611882
a_re484( Apoptosis  ):
PMID:15210107
reviews the differerent hypothesis concerning the biochemical events leading to activation of CASP9
PMID:12773388
in vitro, survivin and HBXIP together inhibits CASP9 processing and activation
in HT1080 cells, coexpression of BIRC5 and HBXIP suppresses staurosporin-induced apoptosis
in cell extracts from Hela transfected with siRNA against HBXIP, CYCS induced caspase activation is suppressed
PMID:10329646
by NOD1
a_re494:( Apoptosis  ) reactionType:is.a

Caspase9*|​S196_unk|​T125_pho|​S144_unk|​Y153_unk@Cytoplasm

References
a_re498( Apoptosis  ):
PMID:12792650
Direct phosphorylation of CASP9 by ERK2 sufficient to block CASP9 processing
in vitro, recombinant hCASP9 phosphorylated on T125 by cytosolic extract or ERK2
in Hela cells, interaction GST-CASP9 / ERK
in Hela or HEK293, CASP9 T125 phosphorylation by OA, consititutively active MEK1, EGF or TPA
PMID:17466630
CASP9 T125 phosphorylation by CCNB1:CDC2
comment: phosphorylation during mitosis, when nuclear envelope is broken down
PMID:19081073
CASP9 T125 phosphorylation by DYRK1A
PMID:19016842
PMID:19586613
CASP9 T125 phosphorylation by DYRK1A and p38
a_re499( Apoptosis  ):
okadaic acid
PMID:16888006
in a murine T cell line stably transfected with the human IL2R, IL2 withdrawal induces PP1 alpha catalytic subunit PP1CA dephosphorylation and activation and CASP9 dephosphorylation

Caspase9*|​S196_unk|​T125_unk|​S144_pho|​Y153_unk@Cytoplasm

References
a_re495:( Apoptosis  ) PMID:16287866

Caspase9*|​S196_pho|​T125_unk|​S144_unk|​Y153_unk@Cytoplasm

References
a_re493( Apoptosis  ):
PMID:9812896
in 267 and MDCK (dog) cell lines
in vitro direct phosphorylation with human recombinant proteins

Caspase9*|​T125_unk|​S144_unk|​Y153_unk@Cytoplasm

References
a_re494:( Apoptosis  ) reactionType:is.a
a_re493( Apoptosis  ):
PMID:9812896
in 267 and MDCK (dog) cell lines
in vitro direct phosphorylation with human recombinant proteins

Caspase9*|​S196_unk|​T125_unk|​Y153_unk@Cytoplasm

References
a_re496:( Apoptosis  ) PMID:16287866
a_re497:( Apoptosis  ) reactionType:is.a

Caspase9*|​S196_unk|​S144_unk|​Y153_unk@Cytoplasm

References
a_re499( Apoptosis  ):
PMID:12792650
okadaic acid
PMID:16888006
in a murine T cell line stably transfected with the human IL2R, IL2 withdrawal induces PP1 alpha catalytic subunit PP1CA dephosphorylation and activation and CASP9 dephosphorylation
a_re500:( Apoptosis  ) reactionType:is.a
a_re498( Apoptosis  ):
Direct phosphorylation of CASP9 by ERK2 sufficient to block CASP9 processing
in vitro, recombinant hCASP9 phosphorylated on T125 by cytosolic extract or ERK2
in Hela cells, interaction GST-CASP9 / ERK
in Hela or HEK293, CASP9 T125 phosphorylation by OA, consititutively active MEK1, EGF or TPA
PMID:17466630
CASP9 T125 phosphorylation by CCNB1:CDC2
comment: phosphorylation during mitosis, when nuclear envelope is broken down
PMID:19081073
CASP9 T125 phosphorylation by DYRK1A
PMID:19016842
PMID:19586613
CASP9 T125 phosphorylation by DYRK1A and p38

Caspase9*|​S196_unk|​T125_unk|​S144_unk|​Y153_unk@Cytoplasm

References
a_re504:( Apoptosis  ) reactionType:is.a
a_re505( Apoptosis  ):
PMID:12620238
cristal structure of the complex
a_re506( Apoptosis  ):
PMID:10671558
in vitro with recombinant proteins
cleavage of recombinant CASP9 by cellular extract, in a Ca2+ dose-dependent manner
in Hela cell extracts, preincubation with Ca2+ and CAPN2 prevents Cyt_c-mediated CASP3 cleavage
in SH-SY5Y human neuroblastoma cells Ca2+ ionophore (A23187) treatment
CAPN2-cleaved CASP9 is inactive
CASP78 are also cleaved by CAPN2, but the effect on their activity is unknown
a_re659:( Apoptosis  ) PMID:15300255
a_re1027( Apoptosis  ):
PMID:14701803
PMID:18497292

Caspase9*|​S196_unk|​T125_unk|​S144_unk|​Y153_unk|​ubi@Cytoplasm

References
a_re659:( Apoptosis  ) PMID:15300255

Caspase9*|​S196_unk|​T125_unk|​S144_unk|​Y153_unk|​-SNO@Cytoplasm

References
a_re1027( Apoptosis  ):
PMID:14701803
PMID:18497292

Caspase9*|​pho@Cytoplasm

References
s_mpk1_re32( Survival  ):
Caspase9 is phosphorylated on Thr125 in a MEK1/2-dependent manner in vivo. Furthermore caspase9 is efficiently phosphorylated on Thr125 by ERK in vitro suggesting that it is targeted directly by ERK in vivo. This is one of the ways ERK plays its anti-apoptotic role.
PMID:12792650

Caspase9*|​pho@Cytoplasm

References
s_akt1_re134:( Survival  ) PMID:9812896

---

Modifications:
In compartment: Cytoplasm

1. Caspase9*@Cytoplasm

2. Caspase9*|​pho@Cytoplasm
3. Caspase9*|​pho@Cytoplasm
4. Caspase9*|​S196_unk|​S144_unk|​Y153_unk@Cytoplasm
5. Caspase9*|​S196_unk|​T125_unk|​S144_unk@Cytoplasm
6. Caspase9*|​S196_unk|​T125_unk|​Y153_unk@Cytoplasm
7. Caspase9*|​T125_unk|​S144_unk|​Y153_unk@Cytoplasm
8. Caspase9*|​S196_pho|​T125_unk|​S144_unk|​Y153_unk@Cytoplasm
9. Caspase9*|​S196_unk|​T125_pho|​S144_unk|​Y153_unk@Cytoplasm
10. Caspase9*|​S196_unk|​T125_unk|​S144_pho|​Y153_unk@Cytoplasm
11. Caspase9*|​S196_unk|​T125_unk|​S144_unk|​Y153_pho@Cytoplasm
12. Caspase9*|​S196_unk|​T125_unk|​S144_unk|​Y153_unk@Cytoplasm
13. Caspase9*|​S196_unk|​T125_unk|​S144_unk|​Y153_unk|​ubi@Cytoplasm
14. Caspase9*|​S196_unk|​T125_unk|​S144_unk|​Y153_unk|​-SNO@Cytoplasm

In compartment: Mitochondrial intermembrane space

1. Caspase9*@Mitochondrial intermembrane space

Participates in complexes:
In compartment: Cytoplasm

1. Caspase9*|​S196_unk|​T125_unk|​S144_unk|​Y153_unk:​XIAP|​S87_unk|​hm2@Cytoplasm

2. Caspase9*:​Cytochrome_C*:​dATP/ATP@Cytoplasm
3. Caspase9*|​hm2:​DRAL*:​PTCH*:​TUCAN*@Cytoplasm

In compartment: Mitochondrial intermembrane space

1. Caspase9*:​HAX1@Mitochondrial intermembrane space

Participates in reactions:
As Reactant or Product:

1. Caspase9*|​S196_unk|​T125_unk|​S144_unk|​Y153_unk@Cytoplasm + NO@Cytoplasm → Caspase9*|​S196_unk|​T125_unk|​S144_unk|​Y153_unk|​-SNO@Cytoplasm

2. Caspase9*@Mitochondrial intermembrane space + HAX1@Mitochondrial intermembrane space → Caspase9*:​HAX1@Mitochondrial intermembrane space
3. Caspase9*@Mitochondrial intermembrane space + H_sub_2_endsub_O_sub_2_endsub_@Mitochondrial intermembrane space → a_s934
4. Caspase9*@Cytoplasm → cleaved~Caspase9*@Cytoplasm
5. Caspase9*|​T125_unk|​S144_unk|​Y153_unk@Cytoplasm → Caspase9*|​S196_pho|​T125_unk|​S144_unk|​Y153_unk@Cytoplasm
6. Caspase9*@Cytoplasm → Caspase9*|​T125_unk|​S144_unk|​Y153_unk@Cytoplasm
7. Caspase9*|​S196_unk|​T125_unk|​Y153_unk@Cytoplasm → Caspase9*|​S196_unk|​T125_unk|​S144_pho|​Y153_unk@Cytoplasm
8. Caspase9*|​S196_unk|​T125_unk|​S144_pho|​Y153_unk@Cytoplasm → Caspase9*|​S196_unk|​T125_unk|​Y153_unk@Cytoplasm
9. Caspase9*@Cytoplasm → Caspase9*|​S196_unk|​T125_unk|​Y153_unk@Cytoplasm
10. Caspase9*|​S196_unk|​S144_unk|​Y153_unk@Cytoplasm → Caspase9*|​S196_unk|​T125_pho|​S144_unk|​Y153_unk@Cytoplasm
11. Caspase9*|​S196_unk|​T125_pho|​S144_unk|​Y153_unk@Cytoplasm → Caspase9*|​S196_unk|​S144_unk|​Y153_unk@Cytoplasm
12. Caspase9*@Mitochondrial intermembrane space → Caspase9*@Cytoplasm
13. Caspase9*@Cytoplasm → Caspase9*|​S196_unk|​S144_unk|​Y153_unk@Cytoplasm
14. Caspase9*|​S196_unk|​T125_unk|​S144_unk@Cytoplasm → Caspase9*|​S196_unk|​T125_unk|​S144_unk|​Y153_pho@Cytoplasm
15. Caspase9*@Cytoplasm → Caspase9*|​S196_unk|​T125_unk|​S144_unk@Cytoplasm
16. Caspase9*|​S196_unk|​T125_unk|​S144_unk|​Y153_pho@Cytoplasm → cleaved~Caspase9*@Cytoplasm
17. Caspase9*@Cytoplasm → Caspase9*|​S196_unk|​T125_unk|​S144_unk|​Y153_unk@Cytoplasm
18. XIAP|​S87_unk@Cytoplasm + Caspase9*|​S196_unk|​T125_unk|​S144_unk|​Y153_unk@Cytoplasm → Caspase9*|​S196_unk|​T125_unk|​S144_unk|​Y153_unk:​XIAP|​S87_unk|​hm2@Cytoplasm
19. Caspase9*|​S196_unk|​T125_unk|​S144_unk|​Y153_unk@Cytoplasm → degraded
20. Caspase9*|​S196_unk|​T125_unk|​S144_unk|​Y153_unk@Cytoplasm → Caspase9*|​S196_unk|​T125_unk|​S144_unk|​Y153_unk|​ubi@Cytoplasm
21. Caspase9*|​S196_unk|​T125_unk|​S144_unk|​Y153_unk|​ubi@Cytoplasm → degraded
22. rCaspase9*@Nucleus → Caspase9*@Cytoplasm
23. Caspase9*@Cytoplasm → Caspase9__sub_TRUNC_endsub_*@Cytoplasm
24. Caspase9*@Cytoplasm → Caspase9*|​pho@Cytoplasm
25. dATP/ATP@Cytoplasm + s_s_akt1_s718 + Cytochrome_C*@Cytoplasm → Caspase9*:​Cytochrome_C*:​dATP/ATP@Cytoplasm
26. Caspase9*@Cytoplasm → Caspase9*|​pho@Cytoplasm
27. PTCH*@Cytoplasm + DRAL*@Cytoplasm + TUCAN*@Cytoplasm + Caspase9*@Cytoplasm → Caspase9*|​hm2:​DRAL*:​PTCH*:​TUCAN*@Cytoplasm

As Catalyser:

1. Caspase9*@Cytoplasm → Caspase9__sub_TRUNC_endsub_*@Cytoplasm

2. Caspase3*@Cytoplasm → Caspase3_sub_TRUNC_endsub_*@Cytoplasm