Human ECT2 (so-called ARHGEF31)is an exchange factor for Rho GTPases, phosphorylated in G2/M phases, and involved in cytokinesis.
The level of GTP-Rho did not change significantly from the S phase to the prometaphase, but increased thereafter, peaking in the telophase, and returned to the original level in the G1 phase.
Expression of the dominant negative form of ECT2 completely suppressed both the rise of GTP-Rho in the telophase and the increased GDP-GTP exchange activity in the mitotic cell extracts.
These results suggest a critical role of ECT2 in Rho activation during cytokinesis
e_re890( EMT ):
GEFs are essential for CDC42 activation. They promote the exchange of GDP to GTP to generate the activated form of CDC42 capable of recognizing downstram targets.
ARHGEF31 as a GEF of CDC42
In compartment: Cytoplasm
Participates in complexes:
Participates in reactions:
As Reactant or Product:
- RhoA_GEFs*@Cytoplasm → ARHGEF31*@Cytoplasm
- Rac1_GEFs*@Cytoplasm → ARHGEF31*@Cytoplasm
- CDC42_GEFs*@Cytoplasm → ARHGEF31*@Cytoplasm