in 293T cells or 293T cells expressing constitutively active Akt, upon over-expression of constitutive active calcineurin (DeltaCnA/CnB)
in HCT116 colon cancer cells, Ca2+ increase (thapsigargin, ionomycin) inhibits BAD S75 phosphorylation and interaction of BAD with 14-3-3 or PP3CB.
in transfected 293T, co-IP BAD phosphomimetic mutant with PPP3CB
in Du145 prostate cancer cells, Ca2+ induced dephosphorylation of BAD correlates with its relocalization to mitochondria
in CSM14.1 immortalized rat hippocampal cells, exogenously expressed BAD-GFP relocalizes to mitochondria in response to cytoplasmic Ca2+ increased (THG, A23187)
in primaryin primary rat hippocampal neurons, L-glutamate induces cell death and relocalization to mitochondria of BAD-GFP, which are both inhibited by co-expression of dominant negative DeltaCnA(H101Q)
in 293T cells or 293T cells expressing mitochondria-targeted RAF1, upon over-expression of constitutive active calcineurin (DeltaCnA/CnB)