a_re631

Reaction state_transition a_re631 map

BAD@Cytoplasm map BAD|​pho@Cytoplasm map

Reaction regulators:

Catalysis
  1. RSK*@Cytoplasm map
  2. S6K*|​pho@Cytoplasm map
  3. AKT*|​S473_pho|​T308_pho@Cytoplasm map
  4. PIM*@Cytoplasm map
  5. PKA_C*@Cytoplasm map
  6. PAK1@Cytoplasm map



References
logical:re631_s3146_re638=FALSE
PMID:9381178
in FL5.12 (murine pro-B-cell lymphoid cell, IL-3 dependent) BAD phosphorylated upon IL-3
in 293T, over-expression of wt Akt or myr-Akt, but not kinase mutant induces phosphorylation of BAD
in vitro phosphorylation of BAD by Akt, BAD S112(human S75)A-S136(human S99)A not phosphorylated
PMID:8929531
2D tryptic peptide mapping identifies S112(human S75) and S136(human S99) as the phospho-sites
phsophorylation in FL5.12 in response to IL-3 or PMA
PMID:9346240
in vitro, purified Akt phosphorylates recombinant BAD at S136(human S99) but not at S112(human S75) (serine mutants)
in Balb/c 3T3 upon PDGF or PC12 upon NGF, BAD phosphorylated in an Akt-dependent manner (wortmannin, LY294002)
partial evidence for BAD:Akt interaction
PMID:10230394
mitochondria localized PKA is a BAD-S112(human S75) kinase
PMID:10558990
in vitro purified activated RSK2/RPS6KA3 phosphorylates a peptide encompassing BAD S112(humanS75) phospho-site. Far less efficient as a BAD-S136 kinase
in 293T, over-expression of RSK2 and MEK1 induces phsophorylation of BAD (anti-BAD pS112)
in BDNF-treated cerebellar granule cells, BDNF induces S112 phosphorylation of BAD, in a MEK1-dependent manner (PD098059)
PMID:10837486
RSK is a BAD S112 kinase (anti BAD-phosphoS112)
in vitro,
in HEK293 upon forskolin TPA isoproterenol or insulin-like growth factor, PKA mediated phosphorylation of S155 of murine BAD (human S118)
S118 lies in the BH3 domain of BAD
phosphorylation of S155 independent of that of S112/136
RSK1-2-3 are BAD S155 kinases
PMID:12954615
in vitro, murine PIM2 immunoprecipitated from trasfected U2OS phosphorylates BAD
in U2OS, PIM2 expression but not that of a kinase dead mutant induces BAD S112 phosphorylation
in FDCP1 murine IL3 dependent hematopoietic cells, upon IL3 withdrawal or doxorubicin, PIM2 ectopic expression is protective
PMID:15280015
BAD S112 phosphorylation by PIM1
PMID:17270021
in SW480 human colon carcinoma cell line, correlation between PIM3 expression and BAD phosphorylation. siRNA against PIM3 abrogates BAD S112 and S136 phosphorylation and induces apoptosis
in a set of human colon cancers, correlation and colocalization of PIM3 and BAD phospho-S112
PMID:11493700
S6K purified as a BAD kinase from FL5.12 BCL-XL/BAD cells
in vitro with recombinant proteins, BAD S136 phosphorylation
in FL5.12 by S6K upon IGF, inhibited by rapamycin
in ES p70S6K-/- cells, IGF-induced BAD S136 phosphorylation is lost
PMID:10611223
in FL5.12 upon IL3, PAK1 phosphorylates BAD on S112 and S136, which results in decreased interaction with BCL2 and BCL2L1, and increased interaction with 14-3-3

Confidence
REF=5 FUNC=4


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